Antibiofilm activity of bromelain from pineapple against Staphylococcus aureus

Bromelain is a set of proteolytic enzymes usually obtained from pineapple (Ananas comosus). Although bromelain has distinguished therapeutic properties, little known about its potential against opportunistic pathogens related to wound healing complications, such as Staphylococcus aureus. This study aimed investigate the antibiofilm and antibacterial activity in 43 clinical strains S. aureus isolated chronic wounds blood cultures. Bromelain’s biofilm vitro was assessed by analyzing formation cultures grown presence 1% destruction after addition mature biofilms. Proteinase K sodium metaperiodate were also added biofilms parallel compare their with that and, together exopolysaccharide protein production rate assays, determine chemical composition extracellular matrix selected evaluated for DNase impact on cellular hydrophobicity auto-aggregation. Mueller-Hinton agar dilution used minimal inhibitory concentration (MIC). Biofilm assays showed significantly inhibits (p = 0.0157) up 4-fold destroys < 0.0001) 6.4-fold, both compared control without bromelain. Biofilms methicillin-resistant most affected treatment. No detected MIC identified main mechanism enzyme, though may contribute. The epithelial properties combined make it promising alternative compose arsenal context care.